Bookedited by Elena García-Fruitós.
Contents:
General introduction : recombinant protein production and purification of insoluble proteins / Neus Ferrer-Miralles ... [et al.]
Overcoming the solubility problem in E. coli : available approaches for recombinant protein production / Agustín Correa and Pablo Oppezzo
Optimization of culture parameters and novel strategies to improve protein solubility / Ranjana Arya ... [et al.]
Cleavable self-aggregating tags (cSAT) for protein expression and purification / Zhanglin Lin ... [et al.]
Beyond the cytoplasm of Escherichia coli : localizing recombinant proteins where you want them / Jason T. Boock ... [et al.]
Characterization of amyloid-like properties in bacterial intracellular aggregates / Anna Villar-Pique, Susanna Navarro, and Salvador Ventura
Co-translational stabilization of insoluble proteins in cell- free expression systems / Lei Kai ... [et al.]
Functional expression of plant membrane proteins in Lactococcus lactis / Sylvain Boutigny .... [et al.]
High cell-density expression system : yeast cells in a phalanx efficiently produce a certain range of "Difficult-to-Express" secretory recombinant proteins / Yasuaki Kawarasaki, Takeshi Kurose, and Keisuke Ito
Insect cells-baculovirus system for the production of difficult to express proteins / Judit Osz-Papai ... [et al.]
Transient expression in HEK 293 cells : an alternative to E. coli for the production of secreted and intracellular mammalian proteins / Joanne E. Nettleship ... [et al.]
Recombinant glycoprotein production in human cell lines / Kamilla Swiech ... [et al.]
Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125 / Maria Giuliani ... [et al.]
Screening methodology for purifying proteins with aggregation problems / Mario Lebendiker, Michal Maes, and Assaf Friedler
Solubilization and refolding of inclusion body proteins / Anupam Singh, Vaibhav Upadhyay, and Amulya K. Panda
Bacterial inclusion body purification / Joaquin Seras-Franzoso ... [et al.]
Characterization of intracellular aggresomes by fluorescent microscopy / Lianwu Fu and Elizabeth Sztul
Dialysis : a characterization method of aggregation tendency / Mireia Pesarrodona, Ugutz Unzueta, and Esther Vázquez
Applications of mass spectrometry to the study of protein aggregation / Sílvia Bronsoms and Sebastián A. Trejo
Insoluble protein assemblies characterized by Fourier transform infrared spectroscopy / Antonino Natalello and Silvia M. Doglia
Insoluble protein characterization by circular dichroism (CD) spectroscopy and nuclear magnetic resonance (NMR) / Shaveta Goyal ... [et al.]
Methods for characterization of protein aggregates / Witold Tatkiewicz ... [et al.]
Predicting the solubility of recombinant proteins in Escherichia coli / Roger G. Harrison and Miguel J. Bagajewicz
Insoluble protein applications : the use of bacterial inclusion bodies as biocatalysts / Eva Hrabárová , Lucia Achbergerová , and Jozef Nahálka.